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Western Blot Antibodies



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Catalog #1: bs-3759R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-3759R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3860R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Hexokinase 1 Polyclonal Antibody, Unconjugated (bs-3992R) at 1:200 followed by conjugation to the secondary antibody
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Hexokinase 1 Polyclonal Antibody, Unconjugated (bs-3992R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-3992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3806R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: FITC

Catalog #1: bs-3806R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Cy3

Catalog #1: bs-3805R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-3647R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3614R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3643R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-3828R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3910R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3669R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3879R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-3669R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3669R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3777R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3577R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3633R-Cy3

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3602R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3495R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:500 overnight at 4\u00b0C, DAB staining.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-RPS6 (Ser235+Ser236) Antibody(bs-3388R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela (H) cells were treated with or without Anisomycin (250ng\/ml) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235+Ser236) polyclonal antibody, unconjugated (bs-3388R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-3388r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3494R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3494R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3543R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3463R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-TAK1(Thr184)Polyclonal Antibody, Unconjugated (bs-3436R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 cell lysates probed with Phospho-TAK1 (Thr184) Polyclonal Antibody, Unconjugated (bs-3436R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3436R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TH (Ser31) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TH (Ser31) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TH (Ser31) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Adrenal gland lysates; Lane 2: Rat Adrenal gland lysates probed with TH (Ser31) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-3463R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3489R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK2\/PYK2 Polyclonal Antibody, Unconjugated (bs-3357R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK2\/PYK2 Polyclonal Antibody, Unconjugated (bs-3357R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3357R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3380R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-3651R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-3513R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3428R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Lane 1: Mouse spleen lysates; Lane 2: Lymph node lysates; Lane 3: Spinal cord lysates probed with RelB Polyclonal Antibody, Unconjugated (bs-3562R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Raji cell lysates; Lane 2: Human Ramos cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with RelB Polyclonal Antibody, Unconjugated (bs-3562R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RelB) Polyclonal Antibody, Unconjugated (bs-3562R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RelB) Polyclonal Antibody, Unconjugated (bs-3562R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3562R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-3352P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti hospho-Paxillin (Tyr118)Polyclonal Antibody, Unconjugated (bs-3352R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 68kD. Observed band size: 68kD.

Catalog #1: bs-3352R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3401R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3559R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-3559R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-3509R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCP3 Polyclonal Antibody, Unconjugated (bs-3509R) at 1:400 overnight at 4\u00b0C, DAB staining.
293T cell lysates probed with CTDSPL Polyclonal Antibody, Unconjugated (bs-3509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3509R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-MEF2A (Ser408)Polyclonal Antibody, Unconjugated (bs-3268R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-3268P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEF2A (Ser408) Polyclonal Antibody, Unconjugated (bs-3268R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Heart lysates probed with MEF2A (Ser408) Polyclonal Antibody, Unconjugated (bs-3268R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3268R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-PLK1 (Ser137) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse large intestine lysates; probed with PLK1 (Ser137) Polyclonal Antibody, unconjugated (bs-3345R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-3345R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-c-Jun(Ser63) Polyclonal Antibody, Unconjugated (bs-3210R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with c-Jun(Ser63) Polyclonal Antibody(bs-3210R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3210R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3396R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3343R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-3395R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3395R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC