RACK1 Polyclonal Antibody

$4% Paraformaldehyde-fixed HepG2(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RACK1) polyclonal Antibody, unconjugated (bs-3647R) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HepG2(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RACK1) polyclonal Antibody, unconjugated (bs-3647R) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Chicken
Rabbit

Overview
Catalog #	bs-3647R
Product Name	RACK1 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RACK1
Immunogen Range	221-317/317
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10399
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	Gnb2 rs1; GNB2L1; Gnb2rs1; Guanine nucleotide binding protein G protein beta polypeptide 2 like 1; Guanine nucleotide binding protein beta polypeptide 2 like 1; Guanine nucleotide binding protein beta subunit 2 like 1; HLC 7; HLC7; Lung cancer oncogene 7; PIG 21; PIG21; proliferation inducing gene 21; Protein homologous to chicken B complex protein guanine nucleotide binding; RACK 1; Receptor for activated C kinase; Receptor of activated protein kinase C 1.
Background	RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific location to form multiprotein complexes, mediating various signal pathways.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200