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Western Blot Antibodies



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Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bs-0127M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-0127m

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0296P

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0296P-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0296P-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0295M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0045M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse placenta lysates probed with Akt1\/3 Polyclonal Antibody, unconjugated (bs-0115M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MAKT1) polyclonal Antibody, Unconjugated (bs-0115M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MAKT1) polyclonal Antibody, Unconjugated (bs-0115M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115M

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0293M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0358M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-0025M

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0297M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0369M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Mouse Anti-Calcineurin A\/PP-2B alpha 1 Polyclonal Antibody (bs-0030M), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Mouse Anti-Calcineurin A\/PP-2B alpha 1 Polyclonal Antibody (bs-0030M), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-0030M

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human HepG2 cell lysates; Lane 2: Human HL-60 cell lysates probed with Monoclonal Polyclonal Antibody, Unconjugated (bsm-33080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33080m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0296P-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0294M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis tissue abeled with Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200 followed by conjugation to the secondary antibody.
Molt-4 lysates probed with PKM2 Polyclonal Antibody, Unconjugated (bs-0102M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Human U251 cell lysates probed with M2-PK Polyclonal Antibody, Unconjugated (bs-0102M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0102M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0297M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium labeled with Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035M) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Kidney lysates; Lane 2: Rat Kidney lysates; Lane 3: Mouse Testis lysates; Lane 4: Rat Testis lysates; Lane 5: Mouse Liver lysates probed with CREB-1 Polyclonal Antibody, Unconjugated (bs-0035M)at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0035M

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0296P-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-4713m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP-Cy7

Catalog #1: bsm-4713m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP

Catalog #1: bsm-4713m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: RBITC

Catalog #1: bsm-4713m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-4713m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PE

Catalog #1: bsm-4713m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5.5

Catalog #1: bsm-4713m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: FITC

Catalog #1: bsm-4713m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy7

Catalog #1: bsm-4713m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5

Catalog #1: bsm-4713m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-750

Catalog #1: bsm-4713m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy3

Catalog #1: bsm-4713m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-647

Catalog #1: bsm-4713m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-594

Catalog #1: bsm-4713m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-405

Catalog #1: bsm-4713m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-680

Catalog #1: bsm-4713m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-350

Catalog #1: bsm-4713m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-488

Catalog #1: bsm-4713m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-555

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-4713m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC-Cy7

Catalog #1: bsm-4713m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC-Cy5.5

Catalog #1: bsm-4713m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC

Catalog #1: bsm-41802m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41802m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41802m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41802m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-41802m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41802m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5