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Western Blot Antibodies



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Catalog #1: bsm-33166M-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bs-0127M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-0127m

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33182M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33281M

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bsm-49131m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-33012M

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33166M

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-33185M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-0225M

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-33281M-BF488

Applications: IF

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-488

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BrdU (A7)) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-0917m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33346m

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-33182M-HRP

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-51003m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:2000~1:10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST tag was immunoprecipitated from GST-tagged mouse S100A7 expression vector whole cell lysate with bsm-33007M at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.\r\n\r\nLane 1: GST-tagged mouse S100A7 expression vector whole cell lysate 5 \u00b5g (Input).\r\nLane 2: bsm-33007M IP in GST-tagged mouse S100A7 expression vector whole cell lysate.\r\nLane 3: native mouse IgG IP in GST-tagged mouse S100A7 expression vector whole cell lysate (negative control).\r\n

Catalog #1: bsm-33007m-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33012M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33011m-hrp-100ul

Applications: WB

Reactivity: Species independent

Conjugate: HRP

20 ng Recombinant RFP protein, His (bs-33011P) per lane probed with RFP monoclonal antibody respectively, unconjugated (bsm-33011M) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
20 ng Recombinant RFP-His protein (bs-33011P) per lane probed with RFP monoclonal antibody respectively, unconjugated (bsm-33011M) at 1:5000-1:50000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33011m-100ul

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-2069M

Applications: WB, ELISA

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-52042m-100ul

Applications: WB, ICC/IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bsm-33003m

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-33346m-hrp-100ul

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #1: bsm-49134m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-33003m-apc-cy7-100ul

Applications: WB

Reactivity: Tags, Others

Conjugate: APC-Cy7

Catalog #1: bsm-33005m-apc-cy7-100ul

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33157m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33185M-Biotin

Applications: WB

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-49133m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-33166M-BF647

Applications: WB

Reactivity: Tags, Others

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33346m-bf647-100ul

Applications: WB

Reactivity: Tags, Others

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33185M-BF350

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33008m-hrp-100ul

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33008m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33007M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33006m-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33166M-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #1: bsm-33003m-hrp-100ul

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #1: bsm-33175M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-49132m-100ul

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bs-0225M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-33011m-bf555-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33007M-BF647

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33007M-PE

Applications: WB

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33189M-HRP

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-0917m-apc-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33006M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33005m-hrp-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33012M-Biotin

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Biotin