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Western Blot Antibodies



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Catalog #1: bs-1557g-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human tumor tissue labeled with Anti-HPV16-E6 Polyclonal Antibody, Unconjugated(bs-0990R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HPV16 E6 protein Polyclonal Antibody, Unconjugated (bs-0990R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with XXX Antibody(bs-R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange). Antibody(bs-0990R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0990R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Unconjugated

Catalog #1: bs-4529R

Applications: WB, IHC-P

Reactivity: Human, NDV

Conjugate: Unconjugated

Antigen: bs-10446P, 0.2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; TMB staining; Read the data in MicroplateReader by 450nm.
Hela (Positive) and RSC96 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha HPV16-E7Antibody (bs-10446R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
Lane 1: Recombinant HPV16 E7 protein Bacterial lysate, DsbC & His(bs-49101L) probed with HPV16 E7 Polyclonal Antibody, Unconjugated (bs-10446R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10446R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Lane 1: Recombinant ADV 5E1A protein, His probed with Adenovirus 5 E1A Polyclonal Antibody, Unconjugated (bs-6136R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
50 ng RAdenovirus 5 E1A-His protein (bs-41297P) per lane probed with Adenovirus 5 E1A polyclonal antibody respectively, unconjugated (bs-6136R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-6136r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-4542R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Virus

Conjugate: Unconjugated

Transfected HeLa cell lysates probed with Anti-CSFV Envelope glycoprotein E2 Polyclonal Antibody, Unconjugated (bs-4527R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P). Probed with CSFV E2 polyclonal Antibody, unconjugated (bs-4527R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4527R

Applications: WB, ELISA

Reactivity: CSFV

Conjugate: Unconjugated

Catalog #1: bs-4504R

Applications: WB, ELISA

Reactivity: PRRSV

Conjugate: Unconjugated

Catalog #1: bs-0271r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4507r

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0130r

Applications: WB, ELISA

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 15ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 10ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 2.5ng probed with SARS-CoV-2 N protein Polyclonal Antibody, Unconjugated (bs-41408R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: SARS-CoV N protein(Cat# bs-49002P) at 50ng; Lane 2: SARS-CoV N protein(Cat# bs-49002P) at 25ng; Lane 3: SARS-CoV N protein(Cat# bs-49002P) at 15ng; Lane 4: SARS-CoV N protein(Cat# bs-49002P) at 10ng ; Lane 5: SARS-CoV N protein(Cat# bs-49002P) at 5ng;Lane 6: SARS-CoV N protein(Cat# bs-49002P) at 2.5ng\uff1bLane 7: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 8: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 9: SARS-CoV-2 N protein(Cat# bs-41408P) at 15ng; Lane 10: SARS-CoV-2 N protein(Cat# bs-41408P) at 10ng ; Lane 11: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng;Lane 12: SARS-CoV-2 N protein(Cat# bs-41408P) at 2.5ng probed with SARS-CoV-2 N protein Polyclonal Antibody, Unconjugated (bs-41408R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-41408r

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2284R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-0746R

Applications: WB

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-6938R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-2022m-100ul

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Virus

Conjugate: Unconjugated

Sample: \r\nhela (Human) Cell Lysate at 30 ug\r\nmcf-7 (Human) Cell Lysate at 30 ug\r\nmda-mb-231 (Human) Cell Lysate at 30 ug\r\nsiha (Human) Cell Lysate at 30 ug\r\nrecombinant HPV16-E7 protein (bs-49101P) 100 ng\r\nrecombinant HPV16-E7 protein (bs-49101P) 50 ng\r\nPrimary: Anti-HPV16 E7 (bs-10202R) at 1\/1000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 11 kD\r\nObserved band size: 17-20 kD\r\nbs-49101P band size: 45 kD
Lane 1: HPV16 E7 fusion protein (bs-49101P) 0.1ug Lysates; Lane 2: HPV16 E7 fusion protein (bs-49101P) 0.05ug Lysates. Probed with HPV16 E7 polyclonal Antibody, unconjugated (bs-10202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HPV16 E7 Polyclonal Antibody(bs-102020R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-10202R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-2968R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-2327R

Applications: WB, ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-2285R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-15457R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-0221R

Applications: WB

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-2147R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-2002R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-0860R

Applications: WB

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-4976R

Applications: WB, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-20116G

Applications: WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: CSFV

Conjugate: Unconjugated

Catalog #1: bs-1674R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-1557G-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: Biotin

Catalog #1: bs-4856R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-4698R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Antigen: bs-2339P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm. \\n
Lane 1: EV71 polyprotein 3D Protein Overexpression E.coli Lysates (Cat#: bs-49067P) probed with EV71 polyprotein 3D Polyclonal Antibody, Unconjugated (bs-2339R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2339R

Applications: WB, ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-4504R-HRP

Applications: WB, ELISA

Reactivity: PRRSV

Conjugate: HRP

Catalog #1: bs-1557G-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: FITC

Catalog #1: bs-10446R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus, Others

Conjugate: Biotin

Catalog #1: bs-0130R-HRP

Applications: WB, ELISA

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-4522R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-2121R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-10446R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus, Others

Conjugate: HRP

Catalog #1: bs-0271R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-10446R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: AbBy Fluor-647

Catalog #1: bs-10202R-HRP

Applications: WB, IHC-P

Reactivity: Virus

Conjugate: HRP

Catalog #1: bsm-2022M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bsm-2022M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Virus

Conjugate: FITC

Catalog #1: bs-4542R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: Biotin

Catalog #1: bs-2284R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-2002R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bs-1690R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated