RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Western Blot Antibodies



×

Collection Menu

Catalog #1: bs-51008r-50ug

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Western blot was performed on whole cell extracts from HeLa cells (25 \u03bcg, lane 1) and recombinant histone H2A, H2B, H3 and H4 (1ug, lane 2, 3, 4 and 5, respectively) using the H3pan (1B2) Monoclonal Antibody (bsm-53008M). The antibody was diluted 1:1,000 in TBSTween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
Western blot was performed on whole cell extracts (30\u03bcg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using H3pan Monoclonal Antibody (bsm-53008M) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
HeLa cells stained with the H3pan Monoclonal Antibody (bsm-53008M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 1% BSA. The cells were labeled with the H3 antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Catalog #1: bsm-53008M

Applications: WB, IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated