TGF alpha Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-TGF alpha Polyclonal Antibody (bs-0066R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat colon labeled with Anti-TGF alpha Polyclonal Antibody Unconjugated (bs-0066R) followed by conjugation to the secondary antibody and DAB staining

Hela cells probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) for 30 minutes followed by incubation with a conjugated secondary (FITC conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Colon tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human U87MG cell lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Colon tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human U87MG cell lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0066R
Product Name	TGF alpha Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TGF-alpha
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7039
Swiss Prot	P01135
Subcellular location	Secreted, Cell membrane, Extracellular matrix
Synonyms	TFGA; Protransforming growth factor alpha; TGFA
Background	TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Application Dilution
WB	1:300-5000
ELISA	1:5000-10000