TNFRSF10A Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti-DR4 Polyclonal Antibody, Unconjugated (bs-0591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti-DR4 Polyclonal Antibody, Unconjugated (bs-0591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DR4 Polyclonal Antibody, Unconjugated (bs-0591R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DR4 Polyclonal Antibody, Unconjugated (bs-0591R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

MCF-7 lysates probed with DR4 Polyclonal Antibody, Unconjugated (bs-0558R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Human Jurkat cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human HeLa cell lysates probed with TNFRSF10A Polyclonal Antibody, Unconjugated (bs-0591R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Jurkat cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human HeLa cell lysates probed with TNFRSF10A Polyclonal Antibody, Unconjugated (bs-0591R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bs-0591R
Product Name	TNFRSF10A Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DR4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8797
Swiss Prot	O00220
Subcellular location	Cell membrane
Synonyms	DR4; APO2; CD261; TRAILR1; TRAILR-1; Tumor necrosis factor receptor superfamily member 10A; Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1; TNFRSF10A
Background	Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Application Dilution
WB	1:300-5000
ELISA	1:5000-10000