ZNF300 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ZNF300 Polyclonal Antibody, Unconjugated (bs-0628R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 rat brain lysates L2 mouse intestine lysates probed with Anti ZNF300 Polyclonal Antibody, Unconjugated (bs-0628R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 66kD. Observed band size:66kD.\\n$

L1 rat brain lysates L2 mouse intestine lysates probed with Anti ZNF300 Polyclonal Antibody, Unconjugated (bs-0628R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 66kD. Observed band size:66kD.\\n

Applications

WB
ELISA
IHC-P

Reactivity

Mouse

Predicted Reactivity

Human

Overview
Catalog #	bs-0628R
Product Name	ZNF300 Polyclonal Antibody
Applications	WB ELISA IHC-P
Reactivity	Mouse
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNF300
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	91975
Swiss Prot	Q96RE9
Subcellular location	Nucleus
Synonyms	Zinc finger protein 3; ZNF3
Background	ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400