Ceramide glucosyltransferase Polyclonal Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti GCS\/glucosylceramide synthase Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti GCS\/glucosylceramide synthase Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Ceramide glucosyltransferase Polyclonal Antibody, Unconjugated (bs-0701R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

$Raji lysates probed with Ceramide glucosyltransferase Polyclonal Antibody, unconjugated (bs-0701R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Raji lysates probed with Ceramide glucosyltransferase Polyclonal Antibody, unconjugated (bs-0701R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded (Human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-0701R
Product Name	Ceramide glucosyltransferase Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GCS
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7357
Swiss Prot	Q16739
Subcellular location	Cytoplasm, Cell membrane
Synonyms	GCS; GLCT1; Ceramide glucosyltransferase; GLCT-1; Glucosylceramide synthase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; UGCG
Background	Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200