TTF1/NKX2-1 Polyclonal Antibody

Independently Validated Antibody, image provided by Science Direct, badge number 028752. Western blot analysis of mouse brain and liver extracts using NK2 Homeobox 1 (bs-0826R NKX2-1) antibody, 1:500 dilution. NKX2-1 is present in the positive control sample (brain) and absent from the negative control sample (liver). The predicted and observed position of NKX2-1 is at around 38kDa.

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody (bs-0826R) at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min. This data was generously submitted by an end user as part of our Bioss Discovery program.

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (bs-0826R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

$Lane 1: Mouse Thyroid gland Lysates; Lane 2: Rat Thyroid gland Lysates. Probed with TTF1\u00a0 polyclonal Antibody, unconjugated (bs-0826R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thyroid gland Lysates; Lane 2: Rat Thyroid gland Lysates. Probed with TTF1\u00a0 polyclonal Antibody, unconjugated (bs-0826R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0826R
Product Name	TTF1/NKX2-1 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TTF-1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7080
Swiss Prot	P43699
Subcellular location	Nucleus
Synonyms	BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; Thyroid-specific enhancer-binding protein; NKX2-1
Background	Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	FCM1:20-100
IHC-F	IFICC1:50-200
IF	ICC1:100-500