MICA Polyclonal Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse lung labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-MICA\/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

A431 cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated (bs-0832R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

$Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Liver tissue lysates probed with MICA Polyclonal Antibody, Unconjugated (bs-0832R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Liver tissue lysates probed with MICA Polyclonal Antibody, Unconjugated (bs-0832R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human

Overview
Catalog #	bs-0832R
Product Name	MICA Polyclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MICA
Immunogen Range	120-170/318
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	100507436
Swiss Prot	Q29983
Subcellular location	Extracellular
Synonyms	MIC-A; PERB11.1; MHC class I polypeptide-related sequence A; MICA
Background	Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF
Flow-Cyt