HGF Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4\u00b0C, DAB staining.

$25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with HGF polyclonal antibody, unconjugated (bs-1025R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit
GuineaPig

Overview
Catalog #	bs-1025R
Product Name	HGF Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HGF alpha
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3082
Swiss Prot	P14210
Subcellular location	Secreted
Synonyms	SF; HGFB; HPTA; F-TCF; DFNB39; Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; HGF
Background	Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200