CD36 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-CD36\/PAS-4 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-CD36\/PAS-4 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Mouse heart lysates probed with Rabbit Anti-CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.$

Mouse heart lysates probed with Rabbit Anti-CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.

$Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human HL-60 cell lysates probed with CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human HL-60 cell lysates probed with CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with CD36 polyclonal antibody, unconjugated (bs-1100R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CD36 polyclonal antibody, unconjugated (bs-1100R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The U937(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD36 antibody (bs-1100R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The U937(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD36 antibody (bs-1100R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-1100r-TR
Product Name	CD36 Polyclonal Antibody
Applications	WB IHC-P IHC-F IP IF FLOW-CYT
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD36
Immunogen Range	51-150/472
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	948
Swiss Prot	P16671
Subcellular location	Cell membrane
Synonyms	BDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3; GPIIIB; IV; PAS; PAS IV; PAS-4; CD36_HUMAN; CD36; Fatty acid translocase (FAT); Glycoprotein IIIb (GPIIIB); Leukocyte differentiation antigen CD36; Platelet collagen receptor; Platelet glycoprotein IV (GPIV); Thrombospondin receptor; CD36_MOUSE; CD36_RAT; Adipocyte membrane protein; Fatty acid translocase; Fatty acid transport protein; CD36 molecule (CD36 blood group); CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); scavenger receptor class B member 3; Platelet glycoprotein 4
Background	Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:50-200
IHC-F	IHC-F=1:50-200
IP	IP=1:20-50
IF	IF=1:50-200
Flow-Cyt	Flow-Cyt=1ug/Test