MRF/C11orf9 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse brain lysate probed with Rabbit Anti-MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 124kD. Observed band size: 124kD$

Mouse brain lysate probed with Rabbit Anti-MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 124kD. Observed band size: 124kD

$Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U2OS cell; Lane 4: Human A431 cell lysates; lysates probed with MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U2OS cell; Lane 4: Human A431 cell lysates; lysates probed with MRF\/C11orf9 Polyclonal Antibody, Unconjugated (bs-11191R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-11191R
Product Name	MRF/C11orf9 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MYRF
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	745
Swiss Prot	Q9Y2G1
Subcellular location	Nucleus, Cell membrane
Synonyms	MRF; Ndt80; pqn-47; C11orf9; Myelin regulatory factor; Myelin gene regulatory factor; MYRF; KIAA0954
Background	Myelin regulatory factor: Consitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes (PubMed:23966832). Myelin regulatory factor, C-terminal: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. Myelin regulatory factor, N-terminal: Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype (PubMed:23966832).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200