Alpha chimerin Polyclonal Antibody

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Sheep
Horse

Overview
Catalog #	bs-11539R
Product Name	Alpha chimerin Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Alpha-chimerin
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm
Synonyms	A-chimaerin; Alpha-chimerin; ARHGAP2; CHIN_HUMAN; CHN; Chn1; N chimaerin; N chimerin; N-chimaerin; N-chimerin; NC; Rho GTPase-activating protein 2; RHOGAP2.
Background	The Rac-GAP chimaerin family member Alpha-chimaerin (also known as N-chimaerin or rho GTPase-activating protein 2) has two splice variants: Alpha1 and Alpha2. The ?-chimaerin variant is a neuron-specific, diacylglycerol-binding and GTPase-activating protein for ras-related protein Rac 1. This variant lacks the N-terminal SH2 domain that is present in the Alpha2 variant. By inactivating Rac 1, Alpha1-chimaerin plays a significant role in the regulation of dendritic growth during neuronal development. It is recruited to the plasma membrane by phospholipase C Beta-coupled cell surface receptors activating the downstream generation of DAG (diacylglycerol). Overexpression of Alpha1-chimaerin results in dendritic spine retraction and the loss of dendritic branches. In the presence of reduced neuronal activity, Alpha1-chimaerin expression is down-regulated resulting in an increase in spine growth and dendritic branching.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200