TRADD Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: human colon carcinoma lysate; Lane 2: rat brain lysate probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.$

Lane 1: human colon carcinoma lysate; Lane 2: rat brain lysate probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.

$Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Cerebrum lysates probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Cerebrum lysates probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1202R
Product Name	TRADD Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse of mouse TRADD
Immunogen Range	111-230/310
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	71609
Swiss Prot	Q3U0V2
Subcellular location	Cytoplasm, Nucleus
Synonyms	AA930854; 9130005N23Rik; Tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; Tradd
Background	Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B (By similarity). The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200