SLC35C2 Polyclonal Antibody

A549 cell lysates probed with SLC35C2 Polyclonal Antibody, Unconjugated (bs-12118R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Large intestine tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with SLC35C2 Polyclonal Antibody, Unconjugated (bs-12118R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Large intestine tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with SLC35C2 Polyclonal Antibody, Unconjugated (bs-12118R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-12118R
Product Name	SLC35C2 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SLC35C2
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cell membrane
Synonyms	BA394O2.1; C20orf5; CGI-15; FLJ37039; MGC20633; MGC32079; MGC39183; Ovarian cancer-overexpressed gene 1 protein; OVCOV1; S35C2_HUMAN; SLC35C2; Solute carrier family 35 member C2.
Background	SLC35C2 (solute carrier family 35, member C2), also known as C20orf5, OVCOV1 or CGI-15, is a 365 amino acid multi-pass membrane protein that belongs to the TPT transporter family and exists as multiple alternatively spliced isoforms. Expressed ubiquitously and functionally induced in hypoxic trophoblast cells, SLC35C2 plays a role in the cellular response to tissue hypoxia and is thought to be involved in the pathogenesis of ovarian cancer. The gene encoding SLC35C2 maps to human chromosome 20, which houses over 600 genes some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. Additionally, chromosome 20 contains a region with numerous genes which are thought important for seminal production and may be potential targets for male contraception.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200