TRAIL Polyclonal Antibody

Formalin-fixed and paraffin embedded rat lung labeled with Anti-TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti-TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Mouse lung lysates; Lane 2: U251 cell lysates probed with Anti\u2013TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.$

Lane 1: Mouse lung lysates; Lane 2: U251 cell lysates probed with Anti\u2013TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1214R
Product Name	TRAIL Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRAIL
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8743
Swiss Prot	P50591
Subcellular location	Cell membrane
Synonyms	TL2; APO2L; CD253; TRAIL; Apo-2L; Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis-inducing ligand; Protein TRAIL; TNFSF10
Background	Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200