TPH Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-TPH\/Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TPH\/Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HL-60 cell lysates probed with Rabbit Anti-Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Rat heart lysates probed with Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with TPH Polyclonal Antibody, Unconjugated (bs-1215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with TPH Polyclonal Antibody, Unconjugated (bs-1215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Horse
Chicken
Rabbit

Overview
Catalog #	bs-1215R
Product Name	TPH Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Tryptophan 5-hydroxylase 1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7166
Swiss Prot	P17752
Subcellular location	Cytoplasm
Synonyms	TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH
Background	This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200