UCMA Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (young rat paw); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (UCMA) Polyclonal Antibody, Unconjugated (bs-12378R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded (young rat paw); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (UCMA) Polyclonal Antibody, Unconjugated (bs-12378R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bs-12378R
Product Name	UCMA Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F
Reactivity	Rat
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UCMA
Immunogen Range	21-120/138
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	221044
Subcellular location	Secreted, Extracellular matrix
Synonyms	C10orf49; Gla rich protein; Gla-rich protein; Grp; GRP; OTTMUSP00000011599; RP23-272I15.1; UCMA; Ucma-C; UCMA_HUMAN; Unique cartilage matrix associated protein; Unique cartilage matrix-associated protein C-terminal fragment; Upper zone of growth plate and cartilage matrix associated.
Background	UCMA is a 138 amino acid secreted protein that is highly expressed in resting chrondrocytes in developing long bones and is thought to function in the early phase of chrondrocyte differentiation. A furin-like protease processes UCMA into an N-terminal 37 amino acid peptide and a C-terminal 74 amino acid peptide, which is referred to as Unique cartilage matrix-associated protein C-terminal fragment (Ucma-C). Introduction of recombinant Ucma-C interferes with osteogenic differentiation of mesenchymal stem cells, MC3T3-E1 preosteoblasts and primary osteoblasts. This suggests that Ucma may be involved in the negative regulation of osteogenic differentiation of osteochondrogenic precursor cells at the cartilage-bone interface and in peripheral zones of fetal cartilage.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500