IGF2BP3 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti IMP3\/IGF-IIBP3 Polyclonal Antibody, Unconjugated (bs-1312R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti IMP3\/IGF-IIBP3 Polyclonal Antibody, Unconjugated (bs-1312R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: HeLa lysates probed with Rabbit Anti-IGF2BP3 Polyclonal Antibody, Unconjugated (bs-1312R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.$

Lane 1: HeLa lysates probed with Rabbit Anti-IGF2BP3 Polyclonal Antibody, Unconjugated (bs-1312R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Horse

Overview
Catalog #	bs-1312R
Product Name	IGF2BP3 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Insulin-like growth factor 2 mRNA-binding protein 3
Immunogen Range	101-230/579
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10643
Swiss Prot	O00425
Subcellular location	Cytoplasm, Nucleus
Synonyms	KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; Insulin-like growth factor 2 mRNA-binding protein 3; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3; IGF2BP3
Background	RNA-binding factor that may recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200