Nogo B Polyclonal Antibody

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Nogo A+B Polyclonal Antibody, Unconjugated (bs-1315R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Cerebellum lysates; Lane 2: Rat Cerebellum lysates probed with Reticulon 4\/Nogo B Polyclonal Antibody, Unconjugated (bs-1315R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebellum lysates; Lane 2: Rat Cerebellum lysates probed with Reticulon 4\/Nogo B Polyclonal Antibody, Unconjugated (bs-1315R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Sheep

Overview
Catalog #	bs-1315R
Product Name	Nogo B Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	Based on the location of the target sequence this antibody will recognize all isoforms(Nogo-A, Nogo-B, and Nogo-C).
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Sheep
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Mouse Reticulon 4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	68585
Swiss Prot	Q99P72
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Reticulon-4; Rtn4; Neurite outgrowth inhibitor; Nogo protein; Foocen; Neuroendocrine-specific protein; NSP; euroendocrine-specific protein C homolog; RTN-x; Reticulon-5; RTN 4A; Nogo-A; RTN-xL; RTN 4B; ASY; Nogo-B; RTN-xS; Foocen-M; RTN 4C; Nogo-C; Foocen-S; RTN4-B2; Rtn-T
Background	Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The reticulon protein is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon 4 regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. May inhibit BACE1 activity and amyloid precursor protein processing.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200