MT2A Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse colon tissue labeled with Anti-Metallothionein Polyclonal Antibody, Unconjugated (bs-1328R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Metallothionein Polyclonal Antibody, Unconjugated (bs-1328R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Metallothionein Polyclonal Antibody, Unconjugated (bs-1328R) at 1:200 overnight at 4\u00b0C, DAB staining.

$U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMetallothionein Polyclonal Antibody(bs-1328R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMetallothionein Polyclonal Antibody(bs-1328R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-1328R
Product Name	MT2A Polyclonal Antibody
Applications	FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F)
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human metallothionein
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	17748
Swiss Prot	P02802
Subcellular location	Cytoplasm, Nucleus
Synonyms	MT-I; Mt-1; Metallothionein-1; Metallothionein-I; Mt1
Background	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200