MBOAT4 Polyclonal Antibody

$Lane 1: Mouse stomach lysates probed with Ghrelin O acyltransferase Antibody, Unconjugated (bs-13355R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37\u02daC.$

Lane 1: Mouse stomach lysates probed with Ghrelin O acyltransferase Antibody, Unconjugated (bs-13355R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (bs-13355R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (bs-13355R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Horse
Rabbit
Monkey
Gorilla

Overview
Catalog #	bs-13355r
Product Name	MBOAT4 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Horse, Rabbit, Monkey, Gorilla
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Ghrelin O acyltransferase
Immunogen Range	101-200/435
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	619373
Swiss Prot	Q96T53
Subcellular location	Cytoplasm, Cell membrane
Synonyms	FKSG89; ghrelin o transferas; Ghrelin O-acyltransferase; GOAT; MBOA4_HUMAN; Mboat4; Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase membrane bound domain containing 4; O-acyltransferase domain-containing protein 4; OACT4.
Background	Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200