Cytokeratin 5 Polyclonal Antibody

$A431 lysates probed with Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1440R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.$

A431 lysates probed with Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1440R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-1440R
Product Name	Cytokeratin 5 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse Cytokeratin 5
Immunogen Range	451-580/580
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3852
Swiss Prot	P13647
Subcellular location	Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms	Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; CK 5; DDD; EBS 2; EBS2; K5; Keratin 5 epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types; Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5.
Background	There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	FCM1:20-100
IF