CD177 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti CD177\/NB1 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti CD177\/NB1 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Hela Cells; Lane 2: K562 Cells; Lane 3: MCF-7 Cells; Lane 4: 293T Cells; Lane 5: Sp2\/0 Cells; Lane 6: NIH\/3T3 Cells; 40ug of lysate loaded into each lane; Probed with CD177 Polyclonal Antibody, unconjugated (bs-1482R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: K562 Cells; Lane 3: MCF-7 Cells; Lane 4: 293T Cells; Lane 5: Sp2\/0 Cells; Lane 6: NIH\/3T3 Cells; 40ug of lysate loaded into each lane; Probed with CD177 Polyclonal Antibody, unconjugated (bs-1482R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Lane 1: Mouse Spleen lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Lymph node lysates probed with CD177 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Mouse
Rat

Overview
Catalog #	bs-1482R
Product Name	CD177 Polyclonal Antibody
Applications	WB IHC-P
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat CD177
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	499099
Swiss Prot	D3ZCH9
Subcellular location	Cell membrane
Synonyms	NB1 GP;PRV-1;HNA-2a;NB1 glycoprotein;Neutrophil Glycoprotein NB1; RGD1562941; CD177 antigen; Polycythemia rubra vera protein 1; PRV-1; NB1 glycoprotein; NB1 GP; Human neutrophil alloantigen 2a; HNA-2a; CD177; CD177_HUMAN; CD177_MOUSE.
Background	Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population. against NB1 can be involved in neonatal alloimmune neutropenia (NAN).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400