C4orf21 Polyclonal Antibody

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-C4orf21 Polyclonal Antibody, Unconjugated (bs-15188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Testis cell lysates; Lane 2: Human MOLT-4 cell lysates; Lane 3: Human HL-60 cell lysates probed with C4orf21 Polyclonal Antibody, Unconjugated (bs-15188R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Testis cell lysates; Lane 2: Human MOLT-4 cell lysates; Lane 3: Human HL-60 cell lysates probed with C4orf21 Polyclonal Antibody, Unconjugated (bs-15188R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Horse

Overview
Catalog #	bs-15188R
Product Name	C4orf21 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic derived from human C4orf21
Immunogen Range	101-200/926
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55345
Swiss Prot	Q86YA3
Subcellular location	Cell membrane
Synonyms	C4orf21; Protein ZGRF1; GRF-type zinc finger domain-containing protein 1; ZGRF1
Background	C4orf12 (chromosome 4 open reading frame 12) is a 926 amino acid single-pass membrane protein that exists as two isoforms. The gene encoding C4orf21 maps to human chromosome 4. Human chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded by a gene that is located on chromosome 4, and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200