IDUA Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain tissue probed with Anti-IDUA Polyclonal Antibody (bs-15542R) at a dilution of 1:200 overnight at 4\u00b0C, followed by Goat Anti-Rabbit secondary, Cy3 conjugated (bs-0295G-Cy3) at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue,C-0033) was used to stain the cell nuclei$

Formalin-fixed and paraffin embedded rat brain tissue probed with Anti-IDUA Polyclonal Antibody (bs-15542R) at a dilution of 1:200 overnight at 4\u00b0C, followed by Goat Anti-Rabbit secondary, Cy3 conjugated (bs-0295G-Cy3) at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue,C-0033) was used to stain the cell nuclei

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with IDUA Polyclonal Antibody, Unconjugated (bs-15542R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with IDUA Polyclonal Antibody, Unconjugated (bs-15542R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA

Reactivity

Human
Mouse

Overview
Catalog #	bs-15542R
Product Name	IDUA Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IDUA
Immunogen Range	101-200/653
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3425
Swiss Prot	P35475
Subcellular location	Cytoplasm
Synonyms	IDA; MPS1; Alpha-L-iduronidase; IDUA
Background	This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
ELISA	1:5000-10000