BBC3/PUMA Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3\/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3\/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (bs-1573R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (bs-1573R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

U87MG cells probed with Puma Polyclonal Antibody, Unconjugated (bs-1573R) at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IP

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Pig

Overview
Catalog #	bs-1573R
Product Name	BBC3/PUMA Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC) IP
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PUMA
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27113
Swiss Prot	Q96PG8
Subcellular location	Cytoplasm
Synonyms	JFY1; PUMA; JFY-1; Bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; BBC3
Background	Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug