phospho-ATP1A1 (Tyr260) Polyclonal Antibody

$Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates probed with phospho-ATP1A1 (Tyr260) Polyclonal Antibody, Unconjugated (bs-15967R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates probed with phospho-ATP1A1 (Tyr260) Polyclonal Antibody, Unconjugated (bs-15967R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha 1 Sodium Potassium ATPase (Tyr260) ) Polyclonal Antibody, Unconjugated (bs-12481R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha 1 Sodium Potassium ATPase (Tyr260) ) Polyclonal Antibody, Unconjugated (bs-12481R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-15967R
Product Name	phospho-ATP1A1 (Tyr260) Polyclonal Antibody
Applications	WB ELISA IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human alpha 1 Sodium Potassium ATPase around the phosphorylation site of Tyr260
Modification Site	Tyr260
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	476
Subcellular location	Cell membrane
Synonyms	alpha 1 Sodium Potassium ATPase phospho Y260; Na,K-ATPase alpha-1; Na+/K+ ATPase 1; Sodium pump 1; ATP1A4; ATP1AL2; ATP1B; ATP1B1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting alpha 4 polypeptide; ATPase Na+/K+ transporting beta 1 polypeptide; MGC1798; MGC25056; MGC3285; MGC51750; Sodium/potassium dependent ATPase beta 1 subunit; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium transporting ATPase subunit beta 1; alpha 1 Sodium Potassium ATPase.
Background	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200