TRAM1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TRAM1 Polyclonal Antibody, Unconjugated (bs-16565R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Human MCF7 cell lysates probed with Anti-TRAM1 Polyclonal Antibody, Unconjugated (bs-16565R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37\u02daC.$

Human MCF7 cell lysates probed with Anti-TRAM1 Polyclonal Antibody, Unconjugated (bs-16565R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-16565R
Product Name	TRAM1 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRAM1
Immunogen Range	61-160/374
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23471
Swiss Prot	Q15629
Subcellular location	Cytoplasm
Synonyms	TRAM; PNAS8; TRAMP; Translocating chain-associated membrane protein 1; TRAM1
Background	Stimulatory or required for the translocation of secretory proteins across the ER membrane.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200