CA II Polyclonal Antibody

Formalin-fixed and paraffin embedded human brain glioblastoma labeled with Anti-CA II Polyclonal Antibody, Unconjugated(bs-1709R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates; Lane 5: Rat Large intestine tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Kidney tissue lysates; Lane 8: Rat Cerebrum tissue lysates probed with CA II Polyclonal Antibody, Unconjugated (bs-1709R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates; Lane 5: Rat Large intestine tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Kidney tissue lysates; Lane 8: Rat Cerebrum tissue lysates probed with CA II Polyclonal Antibody, Unconjugated (bs-1709R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
ELISA
IHC-P

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-1709R
Product Name	CA II Polyclonal Antibody
Applications	WB ELISA IHC-P
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CA II
Immunogen Range	201-260/260
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	760
Swiss Prot	P00918
Subcellular location	Cytoplasm
Synonyms	CAC; CAII; Car2; CA-II; HEL-76; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; Carbonic anhydrase II; CA2
Background	Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400