Orexin Receptor 1 Polyclonal Antibody

$A549 lysates probed with Orexin Receptor 1 Polyclonal Antibody, Unconjugated (bs-18029R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.$

A549 lysates probed with Orexin Receptor 1 Polyclonal Antibody, Unconjugated (bs-18029R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

$Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Pancreas tissue lysates probed with Orexin Receptor 1 Polyclonal Antibody, Unconjugated (bs-18029R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Pancreas tissue lysates probed with Orexin Receptor 1 Polyclonal Antibody, Unconjugated (bs-18029R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-18029R
Product Name	Orexin Receptor 1 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Orexin Receptor type 1
Immunogen Range	101-200/425
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3061
Swiss Prot	O43614
Subcellular location	Cell membrane
Synonyms	Orexin receptor type 1; HCRTR1; Ox-1-R; Ox1R; Hypocretin receptor type 1; HCRTR1
Background	Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide (PubMed:9491897, PubMed:26950369). Triggers an increase in cytoplasmic Ca2+ levels in response to orexin-A binding (PubMed:9491897, PubMed:26950369).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200