Vesicular Acetylcholine Transporter Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-VAT Polyclonal Antibody, Unconjugated (bs-1833R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat brain lysates probed with Rabbit Anti-VAT Polyclonal Antibody, Unconjugated (bs-1833R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human SH-SY5Y cell lysates probed with Vesicular Acetylcholine Transporter Polyclonal Antibody, Unconjugated (bs-1833R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human SH-SY5Y cell lysates probed with Vesicular Acetylcholine Transporter Polyclonal Antibody, Unconjugated (bs-1833R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Rabbit

Overview
Catalog #	bs-1833R
Product Name	Vesicular Acetylcholine Transporter Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VAT
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10493
Swiss Prot	Q99536
Subcellular location	Cell membrane
Synonyms	VATI; Synaptic vesicle membrane protein VAT-1 homolog; VAT1
Background	Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200