NLRP14 Polyclonal Antibody

$Lane 1: DU145 lysates; Lane 2: LOVO lysates probed with NLRP14 Polyclonal Antibody, Unconjugated (bs-19286R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.$

Lane 1: DU145 lysates; Lane 2: LOVO lysates probed with NLRP14 Polyclonal Antibody, Unconjugated (bs-19286R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Ovary tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human SiHa cell lysates; Lane 5: Human JAR cell lysates probed with NLRP14 Polyclonal Antibody, Unconjugated (bs-19286R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Ovary tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human SiHa cell lysates; Lane 5: Human JAR cell lysates probed with NLRP14 Polyclonal Antibody, Unconjugated (bs-19286R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-19286R
Product Name	NLRP14 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NLRP14
Immunogen Range	81-180/1093
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	338323
Swiss Prot	Q86W24
Subcellular location	Cytoplasm
Synonyms	NACHT, LRR and PYD domains-containing protein 14; NLRP14; Nucleotide-binding oligomerization domain protein 5; NOD5
Background	May be involved in inflammation and spermatogenesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200