Phospho-HSD17B13 (Ser33) Polyclonal Antibody

$HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-HSD17B13 (Ser33) Polyclonal Antibody(bs-22308R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-HSD17B13 (Ser33) Polyclonal Antibody(bs-22308R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Lane 1: Human U-2 OS cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U937 cell lysates; Lane 5: Human HepG2 cell lysates probed with Phospho-HSD17B13 (Ser33) Polyclonal Antibody, Unconjugated (bs-22308R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U-2 OS cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U937 cell lysates; Lane 5: Human HepG2 cell lysates probed with Phospho-HSD17B13 (Ser33) Polyclonal Antibody, Unconjugated (bs-22308R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt

Reactivity

Human

Overview
Catalog #	bs-22308r
Product Name	Phospho-HSD17B13 (Ser33) Polyclonal Antibody
Applications	WB FLOW-CYT
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human HSD17B13 around the phosphorylation site of Ser33
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	345275
Swiss Prot	Q7Z5P4
Subcellular location	Secreted
Synonyms	HSD17B13(phospho S33); HSD17B13(phospho Ser33); phospho-HSD17B13(S33); p-HSD17B13(Ser33); 17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; DHB13_HUMAN; HMFN0376; Hsd17b13; SCDR9; Short-chain dehydrogenase/reductase 9; UNQ497/PRO1014.
Application Dilution
WB	=1:500-2000
Flow-Cyt	Flow-Cyt=1ug/Test