Phospho-Cofilin (Ser3) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti Phospho-Cofilin (Ser3) Polyclonal Antibody (bs-3099R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-3099R
Product Name	Phospho-Cofilin (Ser3) Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This phosphorylation site is homologous in the listed cross reactive species at the specified location.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3
Modification Site	Ser3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1072
Swiss Prot	P23528
Subcellular location	Cytoplasm, Nucleus
Synonyms	CFL; cofilin; HEL-S-15; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1
Background	Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200