Phospho-MYPT1 (Thr696) Polyclonal Antibody

Human HeLa cells probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

293T lysates probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Rat BRL-3A lysates probed with p-MYPT1(Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MCF-7 cell lysates probed with Phospho-MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MCF-7 cell lysates probed with Phospho-MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3287R
Product Name	Phospho-MYPT1 (Thr696) Polyclonal Antibody
Applications	WB FCM
Specificity	This phosphorylation site is homologous to that of Thr694 in Mouse and Thr697 in Rat. The region containing this phosphorylation site is known to be homologous to that of the related protein, PP12C, and will react with this protein when phosphorylated at Thr560 in Human and Mouse, and Thr561 in Rat.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696
Modification Site	Thr696
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4659
Swiss Prot	O14974
Subcellular location	Cytoplasm
Synonyms	MBS; M130; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A
Background	Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Application Dilution
WB	1:300-5000
FCM	1:20-100