FCGR1A/CD64 Polyclonal Antibody

$Lane 1: rat lung lysates Lane 2: human colon carcinoma lysates probed with Anti CD64\/IGFR1\/FCGR1A Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 41kD. Observed band size: 41kD.$

Lane 1: rat lung lysates Lane 2: human colon carcinoma lysates probed with Anti CD64\/IGFR1\/FCGR1A Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 41kD. Observed band size: 41kD.

$Lane 1: U937 cell lysates probed with FCGR1A\/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: U937 cell lysates probed with FCGR1A\/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Mouse Raw264.7 cell lysates ; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Liver tissue lysates; Lane 8: Rat Kidney tissue lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human HL60 cell lysates probed with FCGR1A\/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Mouse Raw264.7 cell lysates ; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Liver tissue lysates; Lane 8: Rat Kidney tissue lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human HL60 cell lysates probed with FCGR1A\/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-3511R
Product Name	FCGR1A/CD64 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD64/IGFR1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2209
Swiss Prot	P12314
Subcellular location	Cell membrane
Synonyms	CD64; FCRI; CD64A; IGFR1; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1A; FCG1; FCGR1
Background	High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	ICC1:100-500
IF
ICC/IF