StAR Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 mouse ovary lysates L2 mouse brain lysates probed with Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 32kD. Observed band size:32kD.\\n$

L1 mouse ovary lysates L2 mouse brain lysates probed with Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 32kD. Observed band size:32kD.\\n

Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Lane 1: Mouse Ovary lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human K562cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human U937 cell lysates; Lane 8: Human HepG2 cell lysates probed with StAR Polyclonal Antibody, Unconjugated (bs-3570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Horse

Overview
Catalog #	bs-3570R
Product Name	StAR Polyclonal Antibody
Applications	WB FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human StAR
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6770
Swiss Prot	P49675
Subcellular location	Cytoplasm
Synonyms	STARD1; Steroidogenic acute regulatory protein, mitochondrial; StAR; START domain-containing protein 1
Background	Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200