Phospho-mu Opioid Receptor (Ser375) Polyclonal Antibody

$Antigen: Phospho Mu Opioid Receptor (blue line), 0.2ug\/100ul; Mu Opioid Receptor(red line), 0.2ug\/100ul\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n$

Antigen: Phospho Mu Opioid Receptor (blue line), 0.2ug\/100ul; Mu Opioid Receptor(red line), 0.2ug\/100ul\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n

$Image kindly provided by Dr. Stefen Clemens of East Carolina University. pMOR protein expression levels in the spinal cords of WT and D3KO mice (top lanes), and their respective \u00df-actin protein expression (bottom panels). Rabbit Anti-mu Opioid Receptor (Ser375) Polyclonal Antibody was used at a dilution of 1:1000.$

Image kindly provided by Dr. Stefen Clemens of East Carolina University. pMOR protein expression levels in the spinal cords of WT and D3KO mice (top lanes), and their respective \u00df-actin protein expression (bottom panels). Rabbit Anti-mu Opioid Receptor (Ser375) Polyclonal Antibody was used at a dilution of 1:1000.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-3724R
Product Name	Phospho-mu Opioid Receptor (Ser375) Polyclonal Antibody
Applications	WB IHC-P IHC-F IF(IHC-P)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from rat mu Opioid Receptor around the phosphorylation site of Ser375
Modification Site	Ser375
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25601
Swiss Prot	P33535
Subcellular location	Cell membrane
Synonyms	MORA; Oprm; Oprrm1; Mu-type opioid receptor; M-OR-1; MOR-1; Opioid receptor B; Oprm1; Ror-b
Background	This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200