TMEFF2 Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with TMEFF2 Polyclonal Antibody, Unconjugated (bs-3849R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with TMEFF2 Polyclonal Antibody, Unconjugated (bs-3849R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

$Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (bs-3849R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (bs-3849R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken
Rabbit
GuineaPig

Overview
Catalog #	bs-3849R
Product Name	TMEFF2 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TMEFF2
Immunogen Range	301-374/374
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23671
Subcellular location	Secreted
Synonyms	Tomoregulin-2; HPP 1; HPP1; Hyperplastic polyposis protein 1; TEFF 2; TEFF2; TENB 2; TENB2; Tomoregulin; TPEF; TR; Transmembrane protein containing epidermal growth factor and follistatin domains ; Transmembrane protein TENB2; Transmembrane protein with EGF like and two follistatin like domains 2; TEFF2_HUMAN.
Background	TMEFF2 is a growth factor implicated in the progression of prostate cancer. It is highly expressed in the brain and may act as a survival factor for mesencephalic and hippocampal neurons.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200