phospho-HMGCR (Ser872) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-phospho-HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Cerebrum; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Cerebrum; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: MCF-7 Cells; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

An ELISA was performed using a serial dilution of HMGCR (Ser872) Polyclonal Antibody (bs-4063R) in antigen-coated wells. The antigen used was a peptide containing the phosphosite of interest. The plot shows the absorbance against the antibody dilution for phosphopeptide antigen and non-phosphorylated peptide antigen.

$Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (5% BSA) at room temperature for 30 minutes; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (5% BSA) at room temperature for 30 minutes; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGCR (Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Rabbit

Predicted Reactivity

Dog
Cow
Pig
Chicken

Overview
Catalog #	bs-4063R
Product Name	phospho-HMGCR (Ser872) Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This phosphorylation site is homologous to that of Ser871 in Mouse and Rat.
Reactivity	Human, Mouse, Rat, Rabbit
Predicted Reactivity	Dog, Cow, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human HMGCR around the phosphorylation site of Ser872
Modification Site	Ser872
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3156
Swiss Prot	P04035
Subcellular location	Cytoplasm, Cell membrane
Synonyms	LDLCQ3; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; HMGCR; phospho HMGCR; p-HMGCR
Background	Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200