CHRNB2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CHRNB2 Polyclonal Antibody, Unconjugated (bs-4247R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Cerebral cortex lysates; Lane 4: Rat Cerebrum lysates probed with CHRNB2 Polyclonal Antibody, Unconjugated (bs-4247R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Pig
Chicken

Overview
Catalog #	bs-4247R
Product Name	CHRNB2 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	100% sequence similarity with CHRNB2 and CHRNB4
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHRNB2
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1141, 1143
Subcellular location	Cell membrane
Synonyms	Nicotinic Acetylcholine Receptor beta 2; Acetylcholine receptor beta 2 neural; ACHB2_HUMAN; ACHN; Acrb 2; Acrb2; b2 nAchR; Cholinergic receptor nicotinic beta 2; Cholinergic receptor nicotinic beta polypeptide 2; Cholinergic receptor nicotinic beta polypeptide 2 neuronal; CHRNB2; EFNL 3; EFNL3; nAChRB2; Neuronal acetylcholine receptor protein beta 2 chain precursor; Neuronal acetylcholine receptor protein subunit beta 2; Neuronal acetylcholine receptor subunit beta-2; Neuronal nicotinic acetylcholine receptor beta 2.
Background	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200