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IKK beta Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti- IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti- IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 followed by conjugation to the secondary antibody
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKK_\/_ Polyclonal Antibody(bs-4880R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKK_\/_ Polyclonal Antibody(bs-4880R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-4880R
Product Name IKK beta Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IKK _/_
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3551, 1147
Swiss Prot O14920
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB;I-kappa-B kinase alpha;IKK-A;IKK-alpha; IkBKA; IkappaB kinase; CHUK; Inhibitor of nuclear factor kappa-B kinase; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
Background IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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