Lamin B Receptor Polyclonal Antibody

$Jurkat cell lysates probed with Lamin B Receptor Polyclonal Antibody, Unconjugated (bs-5081R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Jurkat cell lysates probed with Lamin B Receptor Polyclonal Antibody, Unconjugated (bs-5081R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Lamin B Receptor) polyclonal Antibody, Unconjugated (bs-5081R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\r\n\r\n$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Lamin B Receptor) polyclonal Antibody, Unconjugated (bs-5081R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\r\n\r\n

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Horse
Rabbit

Overview
Catalog #	bs-5081R
Product Name	Lamin B Receptor Polyclonal Antibody
Applications	WB ELISA FCM
Specificity	The immunogen for this product has partial sequence similarity to SEPT8 and SEPT2
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Lamin B Receptor
Immunogen Range	1-100/615
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3930
Swiss Prot	Q14739
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	PHA; LMN2R; TDRD18; DHCR14B; Lamin-B receptor; Integral nuclear envelope inner membrane protein; LBR
Background	Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization. Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. The cleavage of lamins results in nuclear disregulation and cell death.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100