MAPK6 Polyclonal Antibody

$L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MAPK6) polyclonal Antibody, Unconjugated (bs-55129R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MAPK6) polyclonal Antibody, Unconjugated (bs-55129R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Lane 1: Hela cell lysates; Lane 2: MAPK6 knockout (KO) HeLa cell lysates probed with MAPK6 Polyclonal Antibody, Unconjugated (bs-55129R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: U-251MG cell lysates; Lane 2: A549 cell lysates; Lane 3: LO2 cell lysates; Lane 4: BxPC-3 cell lysates; Lane 5: 293T cell lysates; Lane 6: Mouse Brain lysates probed with MAPK6 Polyclonal Antibody, Unconjugated (bs-55129R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)
IHC
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55129R
Product Name	MAPK6 Polyclonal Antibody
Applications	WB IF(ICC) IHC IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5597
Swiss Prot	Q16659
Subcellular location	Cytoplasm, Nucleus
Synonyms	MAPK6;ERK3;HsT17250;PRKM6;p97MAPK
Background	The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IHC
IF