S100A11 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Lane 1: BxPC-3 cell lysates; Lane 2: BT-474 cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: SKOV3 cell lysates; Lane 6: THP-1 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: BxPC-3 cell lysates; Lane 2: BT-474 cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: SKOV3 cell lysates; Lane 6: THP-1 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Hela cell lysates; Lane 2: S100A11 knockout (KO) HeLa cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: S100A11 knockout (KO) HeLa cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (S100A11) polyclonal Antibody, Unconjugated (bs-55189R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (S100A11) polyclonal Antibody, Unconjugated (bs-55189R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Human HeLa cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human MCF-7 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:400 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human HeLa cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human MCF-7 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:400 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

$Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (S100A11) Polyclonal Antibody, Unconjugated (bs-55189R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (S100A11) Polyclonal Antibody, Unconjugated (bs-55189R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human breast\r\n carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (S100A11) Polyclonal Antibody, Unconjugated (bs-55189R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human breast\r\n carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (S100A11) Polyclonal Antibody, Unconjugated (bs-55189R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bs-55189r
Product Name	S100A11 Polyclonal Antibody
Applications	WB ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6282
Swiss Prot	P31949
Subcellular location	Cytoplasm, Nucleus
Synonyms	S100A11;HEL-S-43;MLN70;S100C
Background	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
Application Dilution
WB	1:500-2000
ICC/IF	1:50-200