ARHGAP24 Polyclonal Antibody

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with ARHGAP24 Polyclonal Antibody, Unconjugated (bs-5785R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with ARHGAP24 Polyclonal Antibody, Unconjugated (bs-5785R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-5785R
Product Name	ARHGAP24 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ARHGAP24
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	83478
Swiss Prot	Q8N264
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Rho GTPase-activating protein 24; Filamin-A-associated RhoGAP; FilGAP; RAC1- and CDC42-specific GTPase-activating protein of 72 kDa; RC-GAP72; Rho-type GTPase-activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY-SAR-88; p73RhoGAP; ARHGAP24; FILGAP
Background	Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000